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Olea europaea L. subs. oleaster Yapraklarının Süperkritik CO2 Ekstraksiyonuyla Fenolik İçerik ve İn-vitro Antioksidan Aktivitesi

Hatice Banu Keskinkaya,ZELİHA ÜSTÜN ARGON,SÜLEYMAN DOĞU,TURAN AKDAĞ

  • Hatice Banu Keskinkaya: Necmettin Erbakan UNİVERSİTESİ
  • ZELİHA ÜSTÜN ARGON: Necmettin Erbakan UNİVERSİTESİ
  • SÜLEYMAN DOĞU: MERAM MESLEK YÜKSEKOKULU
  • TURAN AKDAĞ: S.Ü. TIP FAK
  •  Yıl : 2023
  •  Cilt : 3
  •  Sayı : 2
  •  Sayfa : 75-85
Bu çalışmada Akdeniz Bölgesi, Mersin ili Tarsus ilçelerinde doğal olarak yetişen ekonomik olarak önemli bir potansiyeli olan Olea europaea L. subs. oleaster (delice zeytin) çeşidine ait zeytin yapraklarının, süperkritik CO2 ekstraksiyon yöntemi (SCFE) ile 150 bar ve 300 bar basınç altında elde edilen ekstraktlarının (sırasıyla OE1 ve OE2) total fenolik (TPC) ve total flavonoid (TFC)  içeriklerinin ve in-vitro antioksidan aktivitelerinin (DPPH•, ABTS•+, CUPRAC ve metal şelatlama aktivitesi) belirlenmesi amaçlanmıştır. Süperkritik ekstrelerin (OE1-OE2) TPC ve TFC değerleri sırasıyla 2418,80±102,1 – 3951,46±123,7 μg GAEs/mg ekstre ve 384,61±16,8 – 491,70±27,3 μg QEs/mg ekstre olarak hesaplandı. DPPH• radikal süpürücü aktivite hariç çalışılan in-vitro antioksidan testlerin hepsinde hem OE1 hem OE2 süperkritik ekstreleri önemli düzeyde aktivite gösterdi.  ABTS•+ radikal süpürme aktivitesi OE2 superkritik ekstresinde (IC50: 30,13±0,82 µg/ml) OE1 superkritik ekstresinden (IC50: 47,21±0,36µg/mL) daha yüksek bulundu. OE2 superkritik ekstresinde CUPRAC aktivitesi (A0.50: 206,52±0,24 µg/mL) OE1 superkritik ekstresinden (A0.50: 256,71±0,13µg/mL) daha yüksek bulundu. Metal kelatlama aktivitesi ise OE2 superkritik ekstresinde (IC50: 26,26±0,72 µg/mL) OE1 superkritik ekstresinden (IC50: 30,17±0,53 µg/mL) daha yüksek gözlendi. DPPH• radikal süpürücü aktivite, O. europaea L.'nin iki ekstraktında da önemli bir aktivite göstermedi. Dolayısıyla süperkritik CO2 ekstraksiyon yönteminde 300 bar basınç altında ekstrakte edilen (OE2) O. europaea subs. oleaster yapraklarının daha yüksek TPC/TFC ve in-vitro antioksidan aktivitelere sahip olduğu gözlendi. Sonuç olarak, O. europaea subs. oleaster yaprak ekstraktlarının fenolik bileşikler açısından çok güçlü bir zenginliğe sahip olduğu ve flavonoidlerin çok ilginç antioksidan potansiyele sahip oldukları gözlendi. Ayrıca gıda koruyucu olarak farklı alanlarda kullanılabilecek önemli fenolik bileşik olabileceğini, sentetik antioksidanlar için O. europaea subs. oleaster yapraklarının önemli bir alternatif oluşturabileceğini düşünüyoruz.
Atıf yapmak için : Keskinkaya, H. B., Üstün Argon, Z., Doğu, S., & Akdağ, T. (2023). Phenolic Content and in-vitro antioxidant activity of olea europaea L. subs. oleaster leaves by supercritical CO2 extraction. Eregli Journal of Agricultural Sciences, 3(2), 75-85. http://dx.doi.org/10.54498/ETBD.2023.25

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Ereğli Tarım Bilimleri Dergisi
2023, Cilt3, Sayı2
E-ISSN: 2822-4167
Geliş Tarihi : , Kabul Tarihi : , Yayın Tarihi :

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