Phenolic Content and In-vitro Antioxidant Activity of Olea europaea L. subs. Oleaster Leaves by Supercritical CO2 Extraction
TURAN AKDAĞ, SÜLEYMAN DOĞU, Hatice Banu Keskinkaya, ZELİHA ÜSTÜN ARGON
- Year : 2023
- Vol : 3
- No : 2
- Page :
75-85
In the study, we aimed to define total phenolic (TPC) and flavonoid (TFC) contents and antioxidant invitro assays (DPPH•, ABTS•+, CUPRAC and metal chelating activity) of supercritical CO2 extracts obtained by supercritical CO2 extraction method (SCFE) under pressure of 150 bar and 300 bar of O. europaea subs. L. oleaster leaf, which grows naturally in the Tarsus district of Mersin province, in the Mediterranean Region, has an important economic potential. TPC and TFC of the supercritical samples (OE1-OE2) were predicted as 2418,80±102,1 – 3951,46±123,7 μg GAEs/mg extract and 384,61±16,8–491,70±27,3 μg QEs, respectively. Except for DPPH• radical scavenging activity, both OE1 and OE2 supercritical extracts showed significant activity in all in-vitro antioxidant tests studied. ABTS•+ radical scavenging activity in the OE2 supercritical extract (IC50: 30,13±0,82 µg/ml) was determined higher than OE1 supercritical extract (IC50: 47,21±0,36µg/ml). CUPRAC activity in OE2 supercritical extract (A0.50: 206,52±0,24 µg/mL) was found higher than OE1 supercritical extract (A0.50: 256,71±0,13µg/mL). Metal chelating activity value of OE2 supercritical extract (IC50: 26,26±0,72 µg/mL) was found higher than OE1 supercritical extract (IC50: 30,17±0,53 µg/mL). DPPH• assay showed not any remarkable activity in extract of O. europaea subs. Oleaster. Therefore, it was observed that O. europaea leaves extracted under 300 bar pressure in the supercritical CO2 extraction method (OE2) had higher TPC/TFC and in-vitro antioxidant activities. As a result, it was observed that O. europaea subs. oleaster leaf extracts had a very strong richness in terms of phenolic compounds and flavonoids had very interesting antioxidant potential. So, we proposed that O. europaea subs. oleaster leaves can be a valuable source of phenolic compounds as a food preservative in different fields, and that O. europaea subs. oleaster leaves can be an important alternative for synthetic antioxidants.
Cite this Article As :
Keskinkaya, H. B., Üstün Argon, Z., Doğu, S., & Akdağ, T. (2023). Phenolic Content and in-vitro antioxidant activity of olea europaea L. subs. oleaster leaves by supercritical CO2 extraction. Eregli Journal of Agricultural Sciences, 3(2), 75-85. http://dx.doi.org/10.54498/ETBD.2023.25
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Phenolic Content and In-vitro Antioxidant Activity of Olea europaea L. subs. Oleaster Leaves by Supercritical CO2 Extraction, Research Article,
2023,
Vol.
3
(2)
Received : 31.10.2023,
Accepted : 11.12.2023
,
Published Online : 31.12.2023
Ereğli Tarım Bilimleri Dergisi
ISSN: ;
E-ISSN: 2822-4167 ;